B.Sc. Botany
5th Semester Syllabus
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MJC-8 (T) : Molecular Biology
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Unit 1: Nucleic acids
◦Structures of DNA and RNA
◦Forms of DNA
◦DNA replication and role of DNA polymerases
◦Different forms of RNA and their role
◦Genetic code
Unit 2: Central dogma, Transcription and Translation in prokaryotes and eukaryotes
◦Central dogma
◦Transcription and Translation in prokaryotes and eukaryotes
Unit 3: Gene regulation in prokaryotes (Lac operon) and eukaryotes
◦Gene regulation in prokaryotes (Lac operon) and eukaryotes
Unit 4: Blotting techniques
◦Northern
◦Southern & western blotting
◦DNA fingerprinting
◦Gel Electrophoresis
◦Polymerase Chain Reaction
Reference Books:
- Watson J.D., Baker, T.A., Bell, S.P., Gann, A., Levine, M., Losick, R. (2007). Molecular Biology of the Gene, Pearson Benjamin Cummings, CSHL Press, New York, U.S.A. 6th edition
- Klug, W.S., Cummings, M.R., Spencer, C.A. (2009). Concepts of Genetics. Benjamin Cummings. U.S.A. 9th edition
- Russell, P. J. (2010). Genetics-A Molecular Approach. Benjamin Cummings, U.S.A. 3rd edition
- Griffiths, A.J.F., Wessler, S.R., Carroll, S.B., Doebley, J. (2010). Introduction to Genetic Analysis, W. H. Freeman and Co., U.S.A. 10th edition
- Karp Gerald (2010). Cell Biology, John Willey and Sons, Inc. 6th edition